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Original Research

Evaluating the Anti-Leech Effects of Methanolic Extracts of Peganum harmala L. and Olea europaea L. on Limnatis nilotica

Bahmani M, Rafieian-Kopaei M, Eftekhari Z, Banihabib E, Hajigholizadeh G, Bahmani F, Azadzadeh J, Abdollahi R, Kheyri A, Sotoudeh A, Karamati SA, Jelodari M.

World Vet. J. 3(2): 33-37, 2016; pii: S232245681300007-3; Cited by (7)

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ABSTRACT

Leeches had several complications such as pain, itching, inflammation, severe anemia, short-term bleeding, hypersensitivity, and anaphylactic reactions in their hosts. Harmal Peganum harmala L. is used as an analgesic and anti-inflammatory agent and it has antibacterial activity. Olive Olea europaea L. has antibacterial, anti-viral, hypoglycemic and the relaxation of blood vessels properties. Antioxidant properties of olive also had been reported. This study was carried out to detect the effects of methanolic extracts of P. harmala L. and O. europaea L. on L. nilotica immature form. In 2011, 55 immature leeches collected from the southern area of Ilam province were prepared. The methanolic extract of O. europaea L and P. harmala L. were compared with levamisole as the control drug. Distilled water was evaluated as the placebo group which investigated L. nilotica using anti-leech assay. Then extract and drugs were added and their effects were screened for 720 min and time to paralyze, kill and death of each leech was recorded. The results showed that olive methanolic extractions (600 and 300mg) could kill the leeches in an average time of 145±77.57 and171±33.28 min, respectively. An average death time for levamisole was found to be 15±7.49 min. The highest effectiveness was found for levamisole at dose 300 mg. Methanol extracts of the Harmal (300 and 600 μg/m) and springs water showed no anti-leech. In sum, olive plant could use for anti Limnatis nilotica expenditure.


Keywords: Peganum harmala L., Olea europaea L., Levamisole, Limnatis nilotica, Anti-leech Assay


This Article Cited By the following articles

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2. Limnatis nilotica infestation in ram and kid in Dehloran city, Ilam province, west of Iran

Asian Pac J Trop Med, Volume 3, Issue 2, April 2013, Pages 155-157.


3. Internal hirudiniasis in a hen (Gallus gallusdomesticus)–The first report in literature
Asian Pac J Trop Med, Volume 3, Issue 3, April 2013, Pages 232-234.


4. Monitoring microbial quality of commercial dairy products in West Azerbaijan province, northwest of Iran

Asian Pac J Trop Med, Volume 4, Supplement 2, September 2014, Pages S824-S829.


5. Antiparasitic herbs used in west regions of Ilam province located in west of Iran
Asian Pac J Trop Med, Volume 4, Supplement 2, September 2014, Pages S764-S769.


6. Preliminary phytochemical investigation and in vitro anthelmintic activities of Maesa lanceolata solvent extracts against Lymnatis nilotica (aquatic leech) 
Journal of Coastal Life Medicine 2016; 4(1): 50-52.


7. The Anti-Leech Effect Of Ethanolic Extract of Achillea Millefolium L. Compared to Levamisole And Niclosamide on Limnatis Nilotica 
Studia Universitatis “Vasile Goldiş”, Seria Ştiinţele Vieţii, Vol. 24, issue 3, 2014, pp.293-297.


How to Cite this Article

Pubmed Style

Bahmani M, Rafieian-Kopaei M, Eftekhari Z, Banihabib E, Hajigholizadeh G, Bahmani F, Azadzadeh J, Abdollahi R, Kheyri A, Sotoudeh A, Karamati SA, Jelodari M. Evaluating the Anti-Leech Effects of Methanolic Extracts of Peganum harmala L. and Olea europaea L. on Limnatis nilotica. World Vet. J. 2016; 3(2): 33-37.


MLA (The Modern Language Association) Style

Bahmani, Mahmoud, et al. "Evaluating the anti-leech effects of methanolic extracts of Peganum harmala L. and Olea europaea L. on Limnatis nilotica." World’s Vet J 3.2 (2013): 33-37.


APA (American Psychological Association) Style

Bahmani, M., Rafieian-Kopaei, M., Eftekhari, Z., Banihabib, E. K., Hajigholizadeh, G. H., Bahmani, F., ... & Karamati, S. A. (2013). Evaluating the anti-leech effects of methanolic extracts of Peganum harmala L. and Olea europaea L. on Limnatis nilotica. World’s Vet J, 3(2), 33-37.


Chicago Style

Bahmani, Mahmoud, Mahmoud Rafieian-Kopaei, Zohre Eftekhari, E. K. Banihabib, G. H. Hajigholizadeh, Farzaneh Bahmani, Jafar Azadzadeh et al. "Evaluating the anti-leech effects of methanolic extracts of Peganum harmala L. and Olea europaea L. on Limnatis nilotica." World’s Vet J 3, no. 2 (2013): 33-37.


Harvard Style

Bahmani, M., Rafieian-Kopaei, M., Eftekhari, Z., Banihabib, E.K., Hajigholizadeh, G.H., Bahmani, F., Azadzadeh, J., Abdollahi, R., Kheyri, A., Sotoudeh, A. and Karamati, S.A., 2013. Evaluating the anti-leech effects of methanolic extracts of Peganum harmala L. and Olea europaea L. on Limnatis nilotica. World’s Vet J, 3(2), pp.33-37.


Vancouver/ICMJE Style

Bahmani M, Rafieian-Kopaei M, Eftekhari Z, Banihabib EK, Hajigholizadeh GH, Bahmani F, Azadzadeh J, Abdollahi R, Kheyri A, Sotoudeh A, Karamati SA. Evaluating the anti-leech effects of methanolic extracts of Peganum harmala L. and Olea europaea L. on Limnatis nilotica. World’s Vet J. 2013;3(2):33-7.