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Volume 14 (3); September 25, 2024
Biochemical Studies on Efficiency of Natural Gum in Chronic Kidney Failure and Liver Cirrhosis in Rats
Lotfy MM, Abdel-Mobdy E, Abdel-Mobdy YE, Salem HM, and Ali HFM.
World Vet. J. 14(3): 293-310, 2024; pii:S232245682400036-14
DOI: https://dx.doi.org/10.54203/scil.2024.wvj36
ABSTRACT: It is well-established that apoptosis, oxidative stress, and inflammation are associated with several disorders, including chronic renal disease and hepatic disease. Oxidative stress (OS) is a major cause of death from end-stage renal disease which also contributes to atherosclerosis and cardiac issues. The present study aimed to assess the efficacy of Gum Arabic (GA) in mitigating renal damage and hepatotoxicity in rats induced by Chloropyrifos-methyl (CPM). A total of 42 male Wistar rats were divided into seven groups, with four groups (group 2 [IC], group 5 [GA1+IC]a, group 6 [GA2+IC], and group 7 [GA1+IC]b treated with CPM for eight weeks to induce hepatic and renal damage. Two models of GA administration, including the standard oral model in drinking water (15% w/v) and the oral model by gavage at a dose of 1 g/kg body weight were administered. Physiological parameters of kidney and liver functions, including urea, creatinine, AST, and ALT along with anti-oxidant factors (Melaodialdehyde, superoxide dismutase, reduced glutathione, and catalase) were measured in plasma, and homogenates of renal and hepatic tissues on day 57 of the experiment. In addition, histopathological examination was conducted on liver and kidney tissues using hematoxylin and eosin stain to evaluate the efficacy of GA on damaged tissues. Gum Arabic was found to significantly reduce CPM toxic effects in the liver and kidney in groups treated with CPM as liver and kidney parameters were reduced to normal levels. Furthermore, GA reduced histological indicators of inflammation, fibrosis, and apoptosis, as well as renal morphological damage. Additionally, it reduced OS in liver and kidney homogenates. In conclusion, GA effectively reduced the damage that CPM inflicted on liver and kidney tissue by stabilizing physiological parameters to normal levels and repairing cellular structures damaged by OS.
Keywords: Antioxidant, Anti-inflammatory, Gum Arabic, Kidney, Liver, Oxidative stress
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Effect of Aloe vera Gel and Sodium Metabisulphite on Expression of Fibroblast Growth Factor in Incision Wound of Rats
Purnamasari YW, Juniarti, Rahmah NA, Ernanda H, and Sari W.
World Vet. J. 14(3): 311-317, 2024; pii:S232245682400037-14
DOI: https://dx.doi.org/10.54203/scil.2024.wvj37
ABSTRACT: An incision wound is a wound caused by being sliced. Two ingredients that play a key role in the wound-healing process are glucomannan and acemannan, which are rich in polysaccharides and growth hormones. Growth hormones stimulate fibroblast activity and proliferation. The present study involved 35 Sprague Dawley male rats, aged 2-3 months old and weighing 200-300 grams. The study comprised seven groups including, negative control group (G1), positive control (aquades, G2), betadine 10% (G3), gel base (0.5 mg, G4), gel base + sodium metabisulfite 0.2 gr (G5), gel base + Aloe vera 5% (G6), and gel base + Aloe vera 5% + sodium metabisulfite 0.2 gr (G7). Each group had five replications. Initially, a 4-cm incision was made on the dorsal skin of each rat. The study lasted 15 days with observations made on days 3, 7, and 15. After the observation period, the rats were anesthetized and then terminated to collect skin tissues for microscopic examination. The tissue samples were then stained immunohistochemically to assess fibroblast growth factor (FGF) expressions. The results showed that the highest FGF expression was observed in the 5% Aloe vera + 2% metabisulfite group (G7), while the lowest FGF expression was in the negative control group (G1). It is concluded that Aloe vera L. extract gel at 5% + 2% metabisulfite (G7) significantly enhances the expression of FGF.
Keywords: Aloe vera L, Fibroblast growth factor, Incision wound, Skin, Sodium metabisulfite
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Determining the Ideal Temperature and Fermentation Duration to Enhance Crude Protein Content and Reduce Crude Fiber in Rice Bran Using Solid-State Fermentation with Aspergillus niger (USM F4)
Animashahun RA, Akpor OB, Olamide MO, Alabi OO, Oyawoye EO, Okocha RC, Oluwafemi P, Animashahun AP, and Idowu A.
World Vet. J. 14(3): 318-327, 2024; pii:S232245682400038-14
DOI: https://dx.doi.org/10.54203/scil.2024.wvj38
ABSTRACT: Solid-state fermentation (SSF) offers a sustainable method for enhancing the nutritional quality of agricultural residues such as red rice bran. This study aimed to determine the optimal temperature and duration for SSF of red rice bran, focusing specifically on increasing the crude protein (CP) content and reducing the crude fiber (CF) content. SFF of rice bran with Aspergillus niger (A. niger) USM F4 was conducted over 14 consecutive days at three different temperatures (25°C, 35°C, and 45°C). A total of 63 samples of rice bran were divided into three temperature groups, each containing 21 samples. Three samples per group were collected at 48-hour intervals over the 14-day fermentation period. The fermentation process for the collected samples at 48-hour intervals was halted by oven drying at 60°C for 24 hours. The fermented products were subjected to proximate analysis for crude protein (CP), ash, ether extract (EE), and crude fiber (CF) contents using the methods outlined by the Association of Official Analytical Chemists (AOAC). The results revealed a significant effect of temperature and fermentation duration on CP, ash, EE, and CF content when compared to the unfermented rice bran kept at room temperature (25oC). The peak values of CP and the highest degradation of CF across all temperature levels were observed on day 10 while the maximum increase in ash and EE content occurred on day 8. Among the temperature conditions, the highest CP values and the lowest CF values were recorded at 35°C. Conversely, the lowest improvements in CP and CF degradation were observed at 25°C on day 10. In conclusion, the optimal conditions for SSF of rice bran with A. niger to enhance CP content and degrade CF are a temperature of 35°C and a fermentation duration of 10 days.
Keywords: Alternate feed resource, Animal production, Aspergillus niger, Proximate component, Solid-state fermentation, Value- addition
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The Effects of Acute Oral Toxicity of Jatropha multifida and Hyptis suaveolens on Zootechnical Parameters in Local Chickens
Sèdégan EBF, Akpo Y, Boko KC, Azalou M, Adoligbé C, Iwaka Ch, Attakpa E, and Alkoiret TI.
World Vet. J. 14(3): 328-337, 2024; pii:S232245682400039-14
DOI: https://dx.doi.org/10.54203/scil.2024.wvj39
ABSTRACT: The substantial use of medicinal plants in traditional poultry farming is a well-established practice. The present study aimed to determine the median lethal dose (LD50) of ethanolic extracts of Jatropha multifida and Hyptis suaveolens in local chickens and to assess the effects of these extracts on feed intake, water intake, and average body weight. The methodology followed OECD Directive 223, which involves administering the highest dose of the extract to the chickens and assessing any mortality. Three homogeneous groups of five chickens each were formed for the limit dose test. The control group received distilled water, while batches 1 and 2 received 700 mg of ethanolic extract of J. multifida and H. suaveolens, respectively. The average body weight of the chickens was 350g ± 20, and the extracts were administered via gavage at a suspension of 2000 mg/kg.bwt of the extract dissolved in water. The results of the phytochemical tests indicated the presence of several chemical compounds known for their therapeutic effects. The productivity of the extract was 2.75 ± 0.19 for J. multifida and 3.3 ± 0.27 for H. suaveolens. After administration of the suspensions, observation for 14 days revealed no mortality. This finding indicated that the LD50 of the utilized ethanolic extracts exceeds the limit dose (2000 mg/kg.bwt). However, feed intake (49 ± 3 > 46±4) and average body weight (436 ± 31 > 388 ± 37) in batch 2 were significantly higher than those in batch 1. Future research should explore the subacute toxicity of J. multifida and H. suaveolens across various chicken breeds.
Keywords: Body weight, Lethal Dose, Local chicken, Medicinal plant, Mortality, Toxicity
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Effects of Butylated Hydroxytoluene and Sorbitol as Diluent Components on Structural and Surface Ultrastructural Changes of Gaga Chicken Sperm During Cryopreservation
Khaeruddin, Ciptadi G, Yusuf M, Suyadi, Natsir MH, Hermadi HA, and Wahjuningsih S.
World Vet. J. 14(3): 338-348, 2024; pii:S232245682400040-14
DOI: https://dx.doi.org/10.54203/scil.2024.wvj40
ABSTRACT: The Gaga chicken is an indigenous Indonesian breed that is important to preserve using semen cryopreservation technology. The study was conducted to determine the effect of adding sorbitol and butylated hydroxytoluene (BHT) in the diluent on the structural and surface ultrastructure of cryopreserved Gaga chicken sperm during cryopreservation /frozen storage. The study aimed to assess how adding sorbitol and butylated hydroxytoluene (BHT) to the diluent affects the structure and surface ultrastructure of cryopreserved Gaga chicken sperm. A completely randomized design was employed with four treatments and 10 replications including egg yolk-lactate ringer diluent (EYLR) as the control group, EYLR diluent with 3 mM BHT, EYLR diluent with 2% sorbitol, and EYLR diluent with both 3 mM BHT and 2% sorbitol. Semen was collected using a massage technique from 4 male chickens aged approximately 10 months, pooled semen was diluted, packaged in 0.25 mL straws, equilibrated for 2 hours at 5 °C, pre-freeze for 10 minutes, frozen for 24 hours, and thawed for 30 seconds at 37 °C. The parameters evaluated were sperm plasma membrane integrity, acrosome integrity, DNA damage, mitochondrial functionality, and surface ultrastructure. The results showed that the treatment had a significant effect on plasma membrane integrity and post-thawing mitochondrial functionality compared to the control, but no effect was observed on acrosome integrity or DNA damage. The results showed that the combination treatment of BHT with sorbitol had a significant effect on plasma membrane integrity and post-thawing mitochondrial function, but did not affect acrosome integrity or DNA damage when compared to the control group. Ultrastructural observations indicated that cryopreservation caused damage to the head, middle, and tail of the sperm in the control groups. However, these changes were prevented by the diluent containing a combination of BHT and sorbitol. The addition of both components (BHT 3 mM + sorbitol 2%) effectively maintained plasma membrane integrity, mitochondrial functionality, and surface ultrastructure of Gaga chicken sperm during cryopreservation.
Keywords: Butylated hydroxytoluene, Chicken sperm, Cryopreservation, Sorbito, Structure, Sperm ultrastructure
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The Pharmacokinetics of Ceftazidime Following its Intravenous Administration in Dogs
Al-Jumaili MA, Al-Abbass NN, and Ibrahim OMS.
World Vet. J. 14(3): 349-354, 2024; pii:S232245682400041-14
DOI: https://dx.doi.org/10.54203/scil.2024.wvj41
ABSTRACT: Ceftazidime is a beta-lactam that is used in the treatment of bacterial infections in humans and companion animals, such as dogs and cats. It is prescribed to treat gram-negative infections, especially those caused by Pseudomonas aeruginosa. This study aimed to compare the pharmacokinetics of ceftazidime using a microbiological assay to evaluate the adequacy of the proposed dosage regimens for susceptible gram-negative bacteria. For this purpose, five healthy mongrel male dogs, with a mean age of four years and an average weight of 19.1 kg, were administered a single intravenous bolus dose of ceftazidime (20 mg/kg). Plasma concentrations were measured using a microbiological assay, and dosage regimens were established by integrating pharmacokinetics data with pharmacodynamics parameters. The results showed that ceftazidime was rapidly distributed to the peripheral tissues (0.189 L/kg), with a half-life of 1.15 hours and a clearance rate of 0.166 L/hr./kg. The results obtained from the pharmacokinetics-pharmacodynamic integration suggested 20 mg/kg q8 hours of ceftazidime for susceptible gram-negative bacteria with a Minimum Inhibitory Concentration of ≤ 8 µg/ml, and 20 mg /kg q12 hours of ceftazidime for susceptible gram-negative bacteria with a Minimum Inhibitory Concentration of ≤ 4 µg/ml. In conclusion, a mild correlation was observed between the dogs’ weight and the ceftazidime half-life, which led to an adjustment of the proposed dosage regimen to 20 mg/kg q8 hours.
Keywords: Ceftazidime, Dog, Dosage regimen, Gram-negative microbe, Pharmacokinetic
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Antibody Response of Mice to Bali Isolate of Canine Parvovirus Propagated in Madin-Darby Canine Kidney Cell Culture
Astawa INM and Kencana GAY.
World Vet. J. 14(3): 355-365, 2024; pii:S232245682400042-14
DOI: https://dx.doi.org/10.54203/scil.2024.wvj42
ABSTRACT: Canine parvovirus (CPV) infection is still common among dogs, leading to severe disease with high mortality. The potential of a local isolate of CPV as an effective vaccine to prevent the disease warrants investigation. This study aimed to determine the antibody response in mice against a Bali isolate of CPV propagated in the Madin-Darby Canine Kidney (MDCK) cell culture. The virus was purified using polyethylene glycol (PEG)-6000 and mixed with an Aluminum hydroxide adjuvant. Fifteen 7-week female mice were divided into three treatment groups: treatment group 1 (PEG-purified virus and Adjuvant), treatment group 2 (crude unpurified virus and adjuvant), and treatment group 3 (adjuvant without virus), with five replicates per group. The Bali isolate of CPV was successfully replicated in MDCK cells, achieving a titer of 210-211 hemagglutination (HA) units after eight serial passages through the cell culture. The virus was confirmed as CPV by immunocytochemistry test using a monoclonal antibody and hemagglutination inhibition (HI) test using chicken anti-CPV polyclonal antibody. Following the first immunization, the antibody endpoint titer in mice immunized with PEG-purified CPV (5.6) was significantly higher than those immunized with crude unpurified CPV (4.2) and adjuvant without CPV (1.4). Similarly, after the second immunization, the antibody endpoint titer in mice immunized with PEG-purified CPV (7.6) also remained significantly higher than those immunized with crude unpurified CPV (6.4) and adjuvant without CPV (0.8). Significant increases in antibody endpoint titer were observed after the second immunization in mice immunized with PEG-purified CPV and crude unpurified CPV, but not in those given adjuvant without CPV. The Bali isolate of CPV propagated in MDCK cell culture induced a robust antibody response in mice, suggesting it’s a potential as an alternative vaccine candidate for preventing CPV infection in dogs.
Keywords: Bali, Canine parvovirus, Madin-Darby Canine Kidney, Mice, Vaccines
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Effects of Two Types of Estrogen on the Follicular Wave for in Vivo Oocyte Collection in Brown Swiss Cows
Payano IU, Eulogio CQ, Gómez EA, Carhuas JN, Villar FA, Fermín JU, and Sánchez NM.
World Vet. J. 14(3): 366-372, 2024; pii:S232245682400043-14
DOI: https://dx.doi.org/10.54203/scil.2024.wvj43
ABSTRACT: The manipulation of follicular waves through hormonal treatments, such as estrogen administration, plays a crucial role in optimizing in-vivo oocyte collection for assisted reproductive technologies. The present study aimed to evaluate the effect of two specific types of estrogen on follicular wave dynamics and their impact on in-vivo oocyte collection in Brown Swiss cows. Fourteen cows, in their first lactation, weighing approximately 340 kg were randomly assigned to one of two treatments including T1 (estradiol cypionate) and T2 (estradiol benzoate). Both treatments were administered at 1.2 mg of estrogen, at day 0 of the experiment. All Brown Swiss cows were provided with a diet entirely consisting of alfalfa grazing. On day 7, follicular wave dynamics were assessed using a DP-50 vet ultrasound device equipped with a 7.5 MHz transducer for transvaginal follicular aspiration guidance. Follicle counts were categorized into three size ranges including 2-4 mm (small), 4-8 mm (medium), and greater than 8 mm (large). Additionally, the quantity and quality (viable oocytes) of the collected oocytes were evaluated by the Ovum Pick Up (OPU) team for oocyte viability on day 7. The study assessed the follicular dynamics (number of follicles) and efficiency of oocyte collection (viable oocytes) in cows treated with Estradiol Cypionate (T1) and Estradiol Benzoate (T2). The average number of small, medium-sized, and large follicles size were 6.048 ± 6.037, 3.16 ± 2.01, and 0.53 ± 0.67 respectively. The total number of follicles was 9.59 ± 3.56. The mean number of viable oocytes recovered was 3.024 ± 1.66, while the mean number of non-viable oocytes was 1.47 ± 1.01. The results indicated no significant differences between treatments in the size of small, medium, and large follicles, nor in the total number of follicles and viable oocytes recovered. However, a significant difference was observed in the number of non-viable oocytes recovered, with a higher mean in T2 (1.86) compared to T1 (1.09). The results indicated an adequate follicular response and viable oocyte recovery in both treatment groups (estradiol cypionate and estradiol benzoate). However, variations in oocyte viability were observed, with estradiol cypionate showing a slight advantage.
Keywords: Follicle count, Follicular wave dynamic, Oocyte collection, Transvaginal follicular aspiration
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Multidrug Resistance in Stray Cats of The North Surabaya Region, East Java, Indonesia
Wibisono FJ, Widiasih DA, Mentari AO, Isnaeni M, Qurratu'ain SH, Jalal I, Islam AF, Al Fardiansyah, and Nguyen-Viet H.
World Vet. J. 14(3): 373-379, 2024; pii:S232245682400044-14
DOI: https://dx.doi.org/10.54203/scil.2024.wvj44
ABSTRACT: Stray cats survive by getting food or drink that is available in the environment, correspondingly, stray cats have relatively high exposure to antibiotic resistance obtained from resistant bacteria found in the environment. The present study was conducted to determine patterns of multidrug resistance and Escherichia coli resistance in stray cats. A total of 50 stray cat anal swab samples were taken randomly from the previously recorded stray cat population in the Surabaya area, East Java, Indonesia. Samples were brought using buffered peptone water. They were cultured on MacConkay Agar differential selective media, and all suspicious colonies of Escherichia coli were examined by biochemical tests. Isolates were then identified, and susceptibility testing was performed according to the Clinical and Laboratory Standards Institute. The results of the resistance test indicated that the multidrug resistance in Escherichia coli bacteria taken from cats was 14.6% (7/48). The high antibiotic resistance of Escherichia coli bacteria in stray cats, which were resistant to many drugs, provides an early warning of environmental health. Environmental health is closely related to animal and human health, especially antibiotic resistance.
Keywords: Antibiotic, Antimicrobial resistance, Escherichia coli, Multidrug resistance, Stray cat
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Efficacy of Avocado Oil Cream in Wound Repair: Macroscopic Analysis, Inflammatory Cells Count, and Collagen Density
Paramanandi DA, Rukyat H, Kristianingrum YP, and Widyarini S.
World Vet. J. 14(3): 380-388, 2024; pii:S232245682400045-14
DOI: https://dx.doi.org/10.54203/scil.2024.wvj45
ABSTRACT: The wound healing process goes through a series of complex stages that are mutually continuous, namely inflammation, proliferation, and maturation. Following wound formation and the release of proinflammatory cytokines and growth factors, inflammation occurs immediately after the hemostasis phase. Proliferation occurs when products processed by the growth factors are present. The final stage, maturation, is portrayed by the plan of the extracellular network. This study utilized oil from avocado fruit (Persea americana), which contains linoleic and oleic acid content that supports skin tissue repair. The study aimed to explore the effects of using avocado oil cream on the area of wound healing, the number of inflammatory cells, and the collagen density. Twenty-four female mice, aged eight weeks, were used as experimental animals by making excision wounds using a 4 mm biopsy punch on the dorsal skin on the left and right sides. The mice were divided into four groups based on the percentage of avocado oil in the topical cream included Group K (control, topical cream without avocado oil), Group P1 (5% topical avocado oil cream), Group P2 (10% topical avocado oil cream), and Group P3 (15% topical avocado oil cream). Macroscopic examination of the wounds was conducted daily on days 3, 6, and 9 after topical cream treatment using a digital caliper. A total of 48 skin tissue samples were collected from days 3, 6, and 9 after cream application, which were then processed for histopathology evaluations using hematoxylin-eosin staining and Masson’s Trichrome staining. Hematoxylin-eosin staining was used to count the inflammatory cells, and Masson's Trichrome staining was employed to assess collagen density. The results revealed that avocado oil had a great impact on wound closure after 9 days of 15% avocado oil cream treatment, reducing the inflammatory cells after 3-6 days of 10% avocado oil cream therapy, and increasing collagen density after 9 days of 15% avocado oil cream application, as compared to the control, non-avocado oil cream group. Avocado oil can help close wounds, reduced the number of inflammatory cells, and increased collagen density when used in topical pharmaceutical formulations. Avocado oil cream may, therefore, be considered a viable option for wound repair treatment.
Keywords: Avocado oil, Collagen, Inflammatory cell, Wound repair
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